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lyn fak fret biosensor plasmid  (Addgene inc)


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    Structured Review

    Addgene inc lyn fak fret biosensor plasmid
    TCEP-induced mild reduction of the cell surface promotes early adhesion–associated FAK activation and focal adhesion assembly. (A) Schematic illustration of the experimental workflow for monitoring FAK activation using <t>a</t> <t>Lyn–FAK</t> <t>FRET</t> biosensor. This schematic was created using BioRender.com . (B) Representative time-lapse FRET ratio images showing ECFP/YPet FRET ratios during early adhesion (Scale bar = 20 μm). (C) Quantification of the time-dependent ECFP/YPet FRET ratio during early adhesion. Data are presented as mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 compared with the Ctrl group. (D) Representative immunofluorescence staining for F-actin (red) and pFAK (green) (Scale bar = 100 μm). (E-J) Quantitative image analysis of cell morphology and focal adhesion (FA) parameters (Scale bar = 50 μm). Data are presented as mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.
    Lyn Fak Fret Biosensor Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lyn fak fret biosensor plasmid/product/Addgene inc
    Average 93 stars, based on 8 article reviews
    lyn fak fret biosensor plasmid - by Bioz Stars, 2026-04
    93/100 stars

    Images

    1) Product Images from "Modulating cell surface chemistry through mild reduction reinforces extracellular-to-intracellular transmission forces and mechano-signaling"

    Article Title: Modulating cell surface chemistry through mild reduction reinforces extracellular-to-intracellular transmission forces and mechano-signaling

    Journal: Materials Today Bio

    doi: 10.1016/j.mtbio.2026.102908

    TCEP-induced mild reduction of the cell surface promotes early adhesion–associated FAK activation and focal adhesion assembly. (A) Schematic illustration of the experimental workflow for monitoring FAK activation using a Lyn–FAK FRET biosensor. This schematic was created using BioRender.com . (B) Representative time-lapse FRET ratio images showing ECFP/YPet FRET ratios during early adhesion (Scale bar = 20 μm). (C) Quantification of the time-dependent ECFP/YPet FRET ratio during early adhesion. Data are presented as mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 compared with the Ctrl group. (D) Representative immunofluorescence staining for F-actin (red) and pFAK (green) (Scale bar = 100 μm). (E-J) Quantitative image analysis of cell morphology and focal adhesion (FA) parameters (Scale bar = 50 μm). Data are presented as mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.
    Figure Legend Snippet: TCEP-induced mild reduction of the cell surface promotes early adhesion–associated FAK activation and focal adhesion assembly. (A) Schematic illustration of the experimental workflow for monitoring FAK activation using a Lyn–FAK FRET biosensor. This schematic was created using BioRender.com . (B) Representative time-lapse FRET ratio images showing ECFP/YPet FRET ratios during early adhesion (Scale bar = 20 μm). (C) Quantification of the time-dependent ECFP/YPet FRET ratio during early adhesion. Data are presented as mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 compared with the Ctrl group. (D) Representative immunofluorescence staining for F-actin (red) and pFAK (green) (Scale bar = 100 μm). (E-J) Quantitative image analysis of cell morphology and focal adhesion (FA) parameters (Scale bar = 50 μm). Data are presented as mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

    Techniques Used: Activation Assay, Immunofluorescence, Staining



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    lyn fak fret biosensor plasmid  (Addgene inc)
    93
    Addgene inc lyn fak fret biosensor plasmid
    TCEP-induced mild reduction of the cell surface promotes early adhesion–associated FAK activation and focal adhesion assembly. (A) Schematic illustration of the experimental workflow for monitoring FAK activation using <t>a</t> <t>Lyn–FAK</t> <t>FRET</t> biosensor. This schematic was created using BioRender.com . (B) Representative time-lapse FRET ratio images showing ECFP/YPet FRET ratios during early adhesion (Scale bar = 20 μm). (C) Quantification of the time-dependent ECFP/YPet FRET ratio during early adhesion. Data are presented as mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 compared with the Ctrl group. (D) Representative immunofluorescence staining for F-actin (red) and pFAK (green) (Scale bar = 100 μm). (E-J) Quantitative image analysis of cell morphology and focal adhesion (FA) parameters (Scale bar = 50 μm). Data are presented as mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.
    Lyn Fak Fret Biosensor Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lyn fak fret biosensor plasmid/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    lyn fak fret biosensor plasmid - by Bioz Stars, 2026-04
    93/100 stars
    		  Buy from Supplier
    lyn fak biosensor  (Addgene inc)
    93
    Addgene inc lyn fak biosensor
    TCEP-induced mild reduction of the cell surface promotes early adhesion–associated FAK activation and focal adhesion assembly. (A) Schematic illustration of the experimental workflow for monitoring FAK activation using <t>a</t> <t>Lyn–FAK</t> <t>FRET</t> biosensor. This schematic was created using BioRender.com . (B) Representative time-lapse FRET ratio images showing ECFP/YPet FRET ratios during early adhesion (Scale bar = 20 μm). (C) Quantification of the time-dependent ECFP/YPet FRET ratio during early adhesion. Data are presented as mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 compared with the Ctrl group. (D) Representative immunofluorescence staining for F-actin (red) and pFAK (green) (Scale bar = 100 μm). (E-J) Quantitative image analysis of cell morphology and focal adhesion (FA) parameters (Scale bar = 50 μm). Data are presented as mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.
    Lyn Fak Biosensor, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lyn fak biosensor/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    lyn fak biosensor - by Bioz Stars, 2026-04
    93/100 stars
    		  Buy from Supplier
    lyn fak  (Addgene inc)
    93
    Addgene inc lyn fak
    TCEP-induced mild reduction of the cell surface promotes early adhesion–associated FAK activation and focal adhesion assembly. (A) Schematic illustration of the experimental workflow for monitoring FAK activation using <t>a</t> <t>Lyn–FAK</t> <t>FRET</t> biosensor. This schematic was created using BioRender.com . (B) Representative time-lapse FRET ratio images showing ECFP/YPet FRET ratios during early adhesion (Scale bar = 20 μm). (C) Quantification of the time-dependent ECFP/YPet FRET ratio during early adhesion. Data are presented as mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 compared with the Ctrl group. (D) Representative immunofluorescence staining for F-actin (red) and pFAK (green) (Scale bar = 100 μm). (E-J) Quantitative image analysis of cell morphology and focal adhesion (FA) parameters (Scale bar = 50 μm). Data are presented as mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.
    Lyn Fak, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lyn fak/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    lyn fak - by Bioz Stars, 2026-04
    93/100 stars
    		  Buy from Supplier
    lynfak  (Addgene inc)
    93
    Addgene inc lynfak
    TCEP-induced mild reduction of the cell surface promotes early adhesion–associated FAK activation and focal adhesion assembly. (A) Schematic illustration of the experimental workflow for monitoring FAK activation using <t>a</t> <t>Lyn–FAK</t> <t>FRET</t> biosensor. This schematic was created using BioRender.com . (B) Representative time-lapse FRET ratio images showing ECFP/YPet FRET ratios during early adhesion (Scale bar = 20 μm). (C) Quantification of the time-dependent ECFP/YPet FRET ratio during early adhesion. Data are presented as mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 compared with the Ctrl group. (D) Representative immunofluorescence staining for F-actin (red) and pFAK (green) (Scale bar = 100 μm). (E-J) Quantitative image analysis of cell morphology and focal adhesion (FA) parameters (Scale bar = 50 μm). Data are presented as mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.
    Lynfak, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lynfak/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    lynfak - by Bioz Stars, 2026-04
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    TCEP-induced mild reduction of the cell surface promotes early adhesion–associated FAK activation and focal adhesion assembly. (A) Schematic illustration of the experimental workflow for monitoring FAK activation using a Lyn–FAK FRET biosensor. This schematic was created using BioRender.com . (B) Representative time-lapse FRET ratio images showing ECFP/YPet FRET ratios during early adhesion (Scale bar = 20 μm). (C) Quantification of the time-dependent ECFP/YPet FRET ratio during early adhesion. Data are presented as mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 compared with the Ctrl group. (D) Representative immunofluorescence staining for F-actin (red) and pFAK (green) (Scale bar = 100 μm). (E-J) Quantitative image analysis of cell morphology and focal adhesion (FA) parameters (Scale bar = 50 μm). Data are presented as mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

    Journal: Materials Today Bio

    Article Title: Modulating cell surface chemistry through mild reduction reinforces extracellular-to-intracellular transmission forces and mechano-signaling

    doi: 10.1016/j.mtbio.2026.102908

    Figure Lengend Snippet: TCEP-induced mild reduction of the cell surface promotes early adhesion–associated FAK activation and focal adhesion assembly. (A) Schematic illustration of the experimental workflow for monitoring FAK activation using a Lyn–FAK FRET biosensor. This schematic was created using BioRender.com . (B) Representative time-lapse FRET ratio images showing ECFP/YPet FRET ratios during early adhesion (Scale bar = 20 μm). (C) Quantification of the time-dependent ECFP/YPet FRET ratio during early adhesion. Data are presented as mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 compared with the Ctrl group. (D) Representative immunofluorescence staining for F-actin (red) and pFAK (green) (Scale bar = 100 μm). (E-J) Quantitative image analysis of cell morphology and focal adhesion (FA) parameters (Scale bar = 50 μm). Data are presented as mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

    Article Snippet: The Lyn–FAK FRET biosensor plasmid (Plasmid #78299, Addgene, Watertown, MA, USA) was inserted into the pAd/CMV/V5-DESTTM GatewayTM vector (V49320, Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer's instructions to generate an adenoviral expression construct.

    Techniques: Activation Assay, Immunofluorescence, Staining